| Gene | Cd177 |
| Chromosome | 7 |
| Deletion Start | 24,760,283 |
| Deletion End | 24,743,477 |
| Deletion Size | 16,807 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 7
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 24,759,393 | 24,759,414 | CACTCAGCCTCCTTCTCTTCTC |
| TUP | 24,759,363 | 24,759,388 | ACTCTTCCCTGCCTCCTGCCTCAGAA |
| TUR | 24,759,320 | 24,759,338 | CAGCTCATGGCTCCAACAC |
| TDF | 24,745,069 | 24,745,088 | AAGCATCCTCCCTCGTCCAG |
| TDP | 24,745,039 | 24,745,061 | ATCCAGCGACACGGAGGGACCTA |
| TDR | 24,745,017 | 24,745,038 | ACCGGCTTAACTCCCAAGTGTC |
PCR Primers
| Primer | Sequence |
| SU | CTGAGGGAAGAGTGTTTACC |
| SD | GATTCTCACCTCCTCCAGTC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | 397 bp | - | + | + |
| TUF | TUR | 95 bp | + | + | - |
| TDF | TDR | 72 bp | + | + | - |
| NeoFwd | SD | 387 bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 12120A-B11 | VGB6 | C57BL/6NTac | |
| 12120A-E8 | VGB6 | C57BL/6NTac | |
| 12120A-F2 | VGB6 | C57BL/6NTac | |
| 12120A-G10 | VGB6 | C57BL/6NTac | |
