12121 Lilra5 Status: ES cell colonies screened / QC one positive
| Gene | Lilra5 |
| Chromosome | 7 |
| Deletion Start | 4,237,761 |
| Deletion End | 4,242,250 |
| Deletion Size | 4,490 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 7
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 4,237,933 | 4,237,954 | TCTCTTCTAGGGCTGAATCTGG |
| TUP | 4,237,957 | 4,237,982 | CAAGAGACCTCCATGTCGGAAGGTGA |
| TUR | 4,237,991 | 4,238,009 | CGAGTGAGGAGCGATCTGG |
| TDF | 4,241,970 | 4,241,990 | GGTCTCAGTACGGACGACTAC |
| TDP | 4,241,992 | 4,242,015 | TCCTGTCCAGAGGAAGCAGACTCC |
| TDR | 4,242,024 | 4,242,046 | ACAGAGGTCAGGGCAGATGAAAG |
PCR Primers
| Primer | Sequence |
| SU | TGAGGTTAAGCGAGATGACC |
| SD | AACCTGACTGATGAGACAGG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 77 bp | + | + | - |
| TDF | TDR | 77 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
