| Gene | Ifitm2 |
| Chromosome | 7 |
| Deletion Start | 140,955,820 |
| Deletion End | 140,954,886 |
| Deletion Size | 935 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 7
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 140,955,655 | 140,955,677 | CTGTGAAGGTGAGTGTGCTAGAG |
| TUP | 140,955,613 | 140,955,642 | AATGGTGTAAGAAGGTGTGTGTGAGCCTGA |
| TUR | 140,955,580 | 140,955,599 | TCACACCTCGGTGCTATCTG |
| TDF | 140,955,187 | 140,955,207 | AGCTCCAGGGCTCCTTACATC |
| TDP | 140,955,154 | 140,955,181 | TCCTTCAGTCTAGGGACAGGAAGATGGT |
| TDR | 140,955,131 | 140,955,149 | CCTGGGCTCCAACCACATC |
PCR Primers
| Primer | Sequence |
| SU | CAAGTCTAGATCCCTGGAAG |
| SD | TGGAGACCAGAAGCCTGAC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 98 bp | + | + | - |
| TDF | TDR | 77 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 12123A-H4 | VGB6 | C57BL/6NTac | |
| 12123A-D9 | VGB6 | C57BL/6NTac | |
| 12123A-A3 | VGB6 | C57BL/6NTac | |
