12127 Tnfsf12 Status: Germline transmission achieved
| Gene | Tnfsf12 |
| Chromosome | 11 |
| Deletion Start | 69,496,426 |
| Deletion End | 69,505,341 |
| Deletion Size | 8,916 |
| Genome Build | 37 |
|
|
Primer Genomic Coordinates on Chromosome 11
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | | | TCTCTGGGAGGTCCTGTTTG |
| TUP | | | CCCTGCCCTTAATTCCTCTAGGAACTGA |
| TUR | | | TCCTGGCTTTCCTCTGTCTG |
| TDF | | | ACTTGCTGGTGAACGGTGTG |
| TDP | | | CCCTGCGCTGCCTGGAAGAATTC
|
| TDR | | | AGGAGAGCTTGCTGCTGTGG |
PCR Primers
| Primer | Sequence |
| SU | TCAGAGTTCTTGGTCTGGAG |
| SD | GCTAGTGAGGTGGATAGTTG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TCATTCTCAGTATTGTTTTGCC |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 12127A-B8 | VGB6 | C57BL/6NTac | |
