12129   Tgfbr1     Status: ES cell colonies screened / QC no positives

GeneTgfbr1
Chromosome4
Deletion Start
Deletion End
Deletion Size
Genome Build37
MGI IDMGI:98728
NCBI Gene ID666236
BAC ID432J21
BAC LibrarybMQ
CassetteTM-ZEN-Ub1
Clones AvailableNo

Sequence Downloads and Strategy Information

  1. 2,000 Bases Upstream (not yet available)
  2. 2,000 Bases Downstream (not yet available)
  3. Primer Sequences
  4. Cassette
  5. Design Diagram
  6. PCR Conditions
  7. Genotyping Strategy

Primer Genomic Coordinates on Chromosome 4

Please be aware that these oligonucleotides have been validated only by Taqman assay.

PrimerStartEndSequence
TUF47,384,20847,384,227AGAAAGCAGTGCCAGGAGAC
TUP47,384,23047,384,257TCAGTGAGATCTGCTATGTCTGCAAGGG
TUR47,384,30247,384,325GAAATTCACTGAGGACAAGGCAAG
TDF47,410,29147,410,309TGGCTGGGACAAGTTGGAG
TDP47,410,31047,410,332TGGGCAGAACCTGTGTTCCAGCA
TDR47,410,34247,410,365GCTATGGTAGAAAGCAGGGAGAAG

PCR Primers

PrimerSequence
SUAGGGCAAATGATTGCCTTATC
SDCAACATTCTGTAACTGTCTGG
LacInRTTGACTGTAGCGGCTGATGTTG
LacInfGGTAAACTGGCTCGGATTAGGG
NeoInRTACTTTCTCGGCAGGAGCAAGGTG
NeoInFTTCGGCTATGACTGGGCACAACAG
LacInZRevGTCTGTCCTAGCTTCCTCACTG
NeoFwdTCATTCTCAGTATTGTTTTGCC

Predicted PCR Products

Forward PrimerReverse PrimerLengthWTHETKO
SULacZRev bp-++
TUFTUR118 bp++-
TDFTDR75 bp++-
NeoFwdSD bp-++
LacInfLacInR210 bp-++
NeoInfNeoInR282 bp-++
VELOCIGENE KOMP DESIGN DIAGRAMS
VelociGene KOMP Design
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VelociGene Genotyping Strategy

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