| Gene | C3 |
| Chromosome | 17 |
| Deletion Start | 57,228,976 |
| Deletion End | 57,204,019 |
| Deletion Size | 24,958 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 17
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 57,227,289 | 57,227,310 | GGCCTGATTACATGGACCTGTC |
| TUP | 57,227,263 | 57,227,284 | TGTCCACTCTGGAAGCCCAGGC |
| TUR | 57,227,232 | 57,227,250 | CCCAGGCTTGGCTGGAATG |
| TDF | 57,203,676 | 57,203,695 | GCCAGGAGAGGAAGCTGGAG |
| TDP | 57,203,650 | 57,203,673 | ACAGATTGCTGTGAGCTGCCCAAA |
| TDR | 57,203,595 | 57,203,615 | TGGCTCAGCAGTAAAGAACAC |
PCR Primers
| Primer | Sequence |
| SU | AGCAGGTACTTTCAAGCTCC |
| SD | TAGCTGACTACTGAGCTCTC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 79 bp | + | + | - |
| TDF | TDR | 101 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 12132A-H9 | VGB6 | C57BL/6NTac | |
| 12132A-G10 | VGB6 | C57BL/6NTac | |
| 12132A-D2 | VGB6 | C57BL/6NTac | |
