12135   Lipg     Status: ES cell colonies screened / QC no positives

GeneLipg
Chromosome18
Deletion Start74,959,274
Deletion End74,939,614
Deletion Size19,661
Genome Build38
MGI IDMGI:1341803
NCBI Gene ID16891
BAC ID404P16
BAC LibrarybMQ
CassetteZEN-Ub1
Clones AvailableNo

Primer Genomic Coordinates on Chromosome 18

Please be aware that these oligonucleotides have been validated only by Taqman assay.

PrimerStartEndSequence
TUF74,960,25474,960,271TGCCCTGGTGGAGTGATG
TUP74,960,22974,960,252CAGGGTCATCCAGAGCCAGCATGA
TUR74,960,18474,960,205AAGTCTTGGTTTGCGTGTTCTG
TDF74,941,78174,941,799AAGGAGCCACCGGGAAGTC
TDP74,941,75374,941,779TGGTGTCCTTAGTCATATGCAGTGCCA
TDR74,941,68374,941,702CCTACGGGAACTCCCTGAAC

PCR Primers

PrimerSequence
SUAAGTACCTCTCCTGGAGTTC
SDAAGCCAGTGCTCACCTAGC
LacInRTTGACTGTAGCGGCTGATGTTG
LacInfGGTAAACTGGCTCGGATTAGGG
NeoInRTACTTTCTCGGCAGGAGCAAGGTG
NeoInFTTCGGCTATGACTGGGCACAACAG
LacInZRevGTCTGTCCTAGCTTCCTCACTG
NeoFwdTCATTCTCAGTATTGTTTTGCC

Predicted PCR Products

Forward PrimerReverse PrimerLengthWTHETKO
SULacZRev bp-++
TUFTUR88 bp++-
TDFTDR117 bp++-
NeoFwdSD bp-++
LacInfLacInR210 bp-++
NeoInfNeoInR282 bp-++
VELOCIGENE KOMP DESIGN DIAGRAMS
VelociGene KOMP Design
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VelociGene Genotyping Strategy

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