12139 Slc27a1 Status: Cancelled
| Gene | Slc27a1 |
| Chromosome | 8 |
| Deletion Start | 71,570,643 |
| Deletion End | 71,586,056 |
| Deletion Size | 15,414 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 8
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 71,571,167 | 71,571,189 | GAGAGGCTTCTGTGGGATTAGTG |
| TUP | 71,571,193 | 71,571,220 | TGACCCACTGTCCAGATTCTTGGAATGA |
| TUR | 71,571,232 | 71,571,250 | CCGCAAGATGCTCCTTCAC |
| TDF | 71,585,501 | 71,585,521 | CCTGTCCAGGGAGTCATTTAC |
| TDP | 71,585,523 | 71,585,552 | TCTTTGTCCTAATTTAGGCCCTCACTCACC |
| TDR | 71,585,568 | 71,585,587 | TTCAGGGTAGGTGGCTGTTG |
PCR Primers
| Primer | Sequence |
| SU | TGATATGGAAGTAGCCAATCC |
| SD | ACACTGGTCCAACTGCAGG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 84 bp | + | + | - |
| TDF | TDR | 87 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
