| Gene | Slc27a2 |
| Chromosome | 2 |
| Deletion Start | 126,553,158 |
| Deletion End | 126,587,943 |
| Deletion Size | 34,786 |
| Genome Build | 38 |
|
|
Primer Genomic Coordinates on Chromosome 2
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 126,553,508 | 126,553,526 | GTGGATCTGGCTGGGACTG |
| TUP | 126,553,529 | 126,553,550 | CAAACTGGGCTGTCCCATGGCG |
| TUR | 126,553,559 | 126,553,581 | GAGACTTGGCACGAATGTTGTAG |
| TDF | 126,587,222 | 126,587,241 | CTTCCACCAGAGCCAGGATG |
| TDP | 126,587,244 | 126,587,266 | CCACCTGAAGCCTTGCTAGTGCC |
| TDR | 126,587,285 | 126,587,304 | TGCCAACAGTGTGCAAAGAG |
PCR Primers
| Primer | Sequence |
| SU | GCGGAGAACTAGATCTCATC |
| SD | CCTCTCTCCAAGAAGCTTAG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 74 bp | + | + | - |
| TDF | TDR | 83 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 12140A-A7 | VGB6 | C57BL/6NTac | |
| 12140A-D9 | VGB6 | C57BL/6NTac | |
| 12140A-H10 | VGB6 | C57BL/6NTac | |
| 12140A-G11 | VGB6 | C57BL/6NTac | |
| 12140A-G3 | VGB6 | C57BL/6NTac | |
| 12140A-D11 | VGB6 | C57BL/6NTac | |
