12144   Cd109     Status: ES cell colonies screened / QC positives

GeneCd109
Chromosome9
Deletion Start78,463,692
Deletion End78,489,894
Deletion Size26,203
Genome Build37
MGI IDMGI:2445221
NCBI Gene ID235505
BAC ID235N12
BAC LibrarybMQ
CassetteZEN-Ub1
Clones AvailableYes

Primer Genomic Coordinates on Chromosome 9

Please be aware that these oligonucleotides have been validated only by Taqman assay.

PrimerStartEndSequence
TUFCCGTAGCATTTCATGGCCTTAC
TUPAAACAGCAGAGGCACCACGTTCACTG
TURCTGCACTGCTCCACTACAC
TDFCGCTGGAAGCACCTTCAC
TDPCGACCCACCCAGGTCTATGTCTCC
TDRGCACTATTGTCACCTGGATTAGAG

PCR Primers

PrimerSequence
SUACAGCGCGACGTTAGCCTG
SDAAAGCAGGTTTGTCCAATCC
LacInRTTGACTGTAGCGGCTGATGTTG
LacInfGGTAAACTGGCTCGGATTAGGG
NeoInRTACTTTCTCGGCAGGAGCAAGGTG
NeoInFTCATTCTCAGTATTGTTTTGCC
LacInZRevGTCTGTCCTAGCTTCCTCACTG
NeoFwdTCATTCTCAGTATTGTTTTGCC

Predicted PCR Products

Forward PrimerReverse PrimerLengthWTHETKO
SULacZRev bp-++
NeoFwdSD bp-++
LacInfLacInR210 bp-++
NeoInfNeoInR282 bp-++

Available Clones

ES Cell ordering information can be found at the KOMP Repository.
Clone NameParental ES CellMouse StrainCassette Copy Number
12144H-A4VGB6C57BL/6NTac
12144H-A12VGB6C57BL/6NTac
12144H-G5VGB6C57BL/6NTac
VELOCIGENE KOMP DESIGN DIAGRAMS
VelociGene KOMP Design
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VelociGene Genotyping Strategy

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