| Gene | Cd109 |
| Chromosome | 9 |
| Deletion Start | 78,615,885 |
| Deletion End | 78,642,087 |
| Deletion Size | 26,203 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 9
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 78,616,720 | 78,616,741 | CCGTAGCATTTCATGGCCTTAC |
| TUP | 78,616,743 | 78,616,768 | AAACAGCAGAGGCACCACGTTCACTG |
| TUR | 78,616,775 | 78,616,793 | CTGCACTGCTCCACTACAC |
| TDF | 78,641,742 | 78,641,759 | CGCTGGAAGCACCTTCAC |
| TDP | 78,641,760 | 78,641,783 | CGACCCACCCAGGTCTATGTCTCC |
| TDR | 78,641,798 | 78,641,821 | GCACTATTGTCACCTGGATTAGAG |
PCR Primers
| Primer | Sequence |
| SU | ACAGCGCGACGTTAGCCTG |
| SD | AAAGCAGGTTTGTCCAATCC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 74 bp | + | + | - |
| TDF | TDR | 80 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 12144H-A4 | VGB6 | C57BL/6NTac | |
| 12144H-A12 | VGB6 | C57BL/6NTac | |
| 12144H-G5 | VGB6 | C57BL/6NTac | |
