12149 Emcn Status: ES cell colonies screened / QC no positives
| Gene | Emcn |
| Chromosome | 3 |
| Deletion Start | 137,372,748 |
| Deletion End | 137,391,696 |
| Deletion Size | 18,949 |
| Genome Build | 38 |
|
|
Primer Genomic Coordinates on Chromosome 3
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 137,372,985 | 137,373,006 | TCTGAGTAGAACGTAGCGTCTG |
| TUP | 137,373,008 | 137,373,035 | AGGCCTTGCTGAGTTTCCTTACTGATGC |
| TUR | 137,373,046 | 137,373,068 | GGAGTGTTCCCAAACAGGAAGTG |
| TDF | 137,391,083 | 137,391,106 | TGATCTGGCTTGTGTATGTGTATG |
| TDP | 137,391,110 | 137,391,135 | TGCATTCTTGGGAAGCAAGAGAGCCA |
| TDR | 137,391,143 | 137,391,164 | AGAAGAGGCAGCAGCTAAAGAG |
PCR Primers
| Primer | Sequence |
| SU | AAATGGACCCAGGAGCTAAG |
| SD | TTTGCATTGACACCCACACC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 84 bp | + | + | - |
| TDF | TDR | 82 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
