12150 Adm Status: ES cell colonies screened / QC positives
| Gene | Adm |
| Chromosome | 7 |
| Deletion Start | 117,771,797 |
| Deletion End | 117,772,690 |
| Deletion Size | 894 |
| Genome Build | 37 |
|
|
Primer Genomic Coordinates on Chromosome 7
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | | | GTCGTGGGAAGAGGGAAC |
| TUP | | | CAAGCATCCAGCAGCTACCCTACGG |
| TUR | | | CAAGAGTCTGGGTAGGAACTGTC |
| TDF | | | GAAGCCCACATTCGTGTCAAAC |
| TDP | | | CTACCGCCAGAGCATGAACCAGGG
|
| TDR | | | CCCGAAGCGGCATCCATTG |
PCR Primers
| Primer | Sequence |
| SU | GCACTTCTCAATGGCTTCCT |
| SD | ATGAGGCTTATGCCCCGTG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TCATTCTCAGTATTGTTTTGCC |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 12150A-C5 | VGB6 | C57BL/6NTac | |
| 12150A-E3 | VGB6 | C57BL/6NTac | |
| 12150A-F10 | VGB6 | C57BL/6NTac | |
| 12150A-D2 | VGB6 | C57BL/6NTac | |
