| Gene | Vcam1 |
| Chromosome | 3 |
| Deletion Start | 116,128,871 |
| Deletion End | 116,110,346 |
| Deletion Size | 18,526 |
| Genome Build | 38 |
|
|
Primer Genomic Coordinates on Chromosome 3
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 116,128,702 | 116,128,721 | GCTGCGAGTCACCATTGTTC |
| TUP | 116,128,675 | 116,128,701 | TCATGGAGAACCCAGATAGACAGCCCA |
| TUR | 116,128,654 | 116,128,674 | CGTCCTCACCTTCGCGTTTAG |
| TDF | 116,111,460 | 116,111,484 | CAACCATCAACAAGAATGCTCTACC |
| TDP | 116,111,422 | 116,111,448 | TGGCCACACTGAGTGTTGCATAGATTG |
| TDR | 116,111,387 | 116,111,410 | GCCATGAACAGAAATGCCTAACAC |
PCR Primers
| Primer | Sequence |
| SU | GGACTCAGAATGACTTCAGC |
| SD | ACATCATGGGACCATTCCAG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 68 bp | + | + | - |
| TDF | TDR | 98 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 12152A-A7 | VGB6 | C57BL/6NTac | |
| 12152A-B9 | VGB6 | C57BL/6NTac | |
| 12152A-F6 | VGB6 | C57BL/6NTac | |
| 12152A-B5 | VGB6 | C57BL/6NTac | |
