12155 Scn10a Status: ES cell colonies screened / QC no positives
| Gene | Scn10a |
| Chromosome | 9 |
| Deletion Start | 119,638,042 |
| Deletion End | 119,608,505 |
| Deletion Size | 29,538 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 9
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 119,694,287 | 119,694,307 | TCCGTGGGAACTACCAACTTC |
| TUP | 119,694,261 | 119,694,284 | ACGGTTCACTCCAGAGTCGCTGGC |
| TUR | 119,694,204 | 119,694,224 | TGCTTAGGTCTGCCCTTCTTG |
| TDF | 119,665,051 | 119,665,071 | GTACCCTAACCCTCAACCTTC |
| TDP | 119,665,022 | 119,665,049 | TGGTCTCAGATCCCTTATCTCCATGGCC |
| TDR | 119,664,989 | 119,665,011 | ACTGATCTTGGTGTTTGCCTGTC |
PCR Primers
| Primer | Sequence |
| SU | TAGATGGACTGCAGAGGATG |
| SD | AGTCACCCTGTATCTGCTTC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 104 bp | + | + | - |
| TDF | TDR | 83 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
