12156 Scn11a Status: ES cell colonies screened / QC no positives
| Gene | Scn11a |
| Chromosome | 9 |
| Deletion Start | 119,781,967 |
| Deletion End | 119,757,637 |
| Deletion Size | 24,331 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 9
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 119,819,473 | 119,819,491 | GAGCGTGTGGTCAGTCAGG |
| TUP | 119,819,449 | 119,819,471 | CAGGAGGTCAGGCCACTGCTTGA |
| TUR | 119,819,423 | 119,819,444 | GGGTAGCCATTTGTCTGGAGTC |
| TDF | 119,796,625 | 119,796,643 | GCCCAGGGCTAAGGGAATG |
| TDP | 119,796,595 | 119,796,621 | TCCCAAACCTGACCACCGTAATTTACC |
| TDR | 119,796,573 | 119,796,593 | TCCCACCACAAGGATCTCAAG |
PCR Primers
| Primer | Sequence |
| SU | AAGTCTGTCTGTTCCTCCTG |
| SD | AGTACACCTCTCAGGAAAGC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 69 bp | + | + | - |
| TDF | TDR | 71 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
