12159 Scn9a Status: Cancelled
| Gene | Scn9a |
| Chromosome | 2 |
| Deletion Start | 66,568,261 |
| Deletion End | 66,546,982 |
| Deletion Size | 21,280 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 2
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 66,567,579 | 66,567,597 | TCTGGGCAGCTACTTGTGG |
| TUP | 66,567,550 | 66,567,577 | AATACGTTGAGCACAGAGGTCAGAAGGA |
| TUR | 66,567,523 | 66,567,545 | GTTGCTCTGCTTTCTTGAACCTC |
| TDF | 66,547,172 | 66,547,195 | TCTTTGTCGTGGTGATATTTCTGG |
| TDP | 66,547,134 | 66,547,163 | TACCTGATAAACTTGATCCTGGCTGTGGTA |
| TDR | 66,547,098 | 66,547,118 | GATGTTGGCCTGGTTCTGTTC |
PCR Primers
| Primer | Sequence |
| SU | GAGTGTAGAGTCTTGTCTCC |
| SD | AGATTGCTGTGTGAGGCTAG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 75 bp | + | + | - |
| TDF | TDR | 98 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
