12160   Ocstamp     Status: ES cell colonies screened / QC no positives

GeneOcstamp
Chromosome2
Deletion Start
Deletion End
Deletion Size
Genome Build37
MGI IDMGI:1921864
NCBI Gene ID74614
BAC ID206P4
BAC LibrarybMQ
CassetteZEN-Ub1
Clones AvailableNo

Sequence Downloads and Strategy Information

  1. 2,000 Bases Upstream (not yet available)
  2. 2,000 Bases Downstream (not yet available)
  3. Primer Sequences
  4. Cassette
  5. Design Diagram
  6. PCR Conditions
  7. Genotyping Strategy

Primer Genomic Coordinates on Chromosome 2

Please be aware that these oligonucleotides have been validated only by Taqman assay.

PrimerStartEndSequence
TUF165,399,571165,399,590TGAAGCCAGCCATCGTGTAG
TUP165,399,546165,399,569CTCCCGCCTGCTGTAACTGGTGTA
TUR165,399,497165,399,518GCAGGAAGGGAAGTGGATAGAC
TDF165,395,874165,395,893GGACCTCAGGCACATGACTC
TDP165,395,850165,395,873CCTTGGGCCTCCATATGACCTCGA
TDR165,395,786165,395,808TGTTGCCACGCCCAATTATTATG

PCR Primers

PrimerSequence
SUCACTGCTTGGAAAGTGTGTC
SDACGTGAATTCTCAAAGGCTTG
LacInRTTGACTGTAGCGGCTGATGTTG
LacInfGGTAAACTGGCTCGGATTAGGG
NeoInRTACTTTCTCGGCAGGAGCAAGGTG
NeoInFTTCGGCTATGACTGGGCACAACAG
LacInZRevGTCTGTCCTAGCTTCCTCACTG
NeoFwdTCATTCTCAGTATTGTTTTGCC

Predicted PCR Products

Forward PrimerReverse PrimerLengthWTHETKO
SULacZRev bp-++
TUFTUR94 bp++-
TDFTDR108 bp++-
NeoFwdSD bp-++
LacInfLacInR210 bp-++
NeoInfNeoInR282 bp-++
VELOCIGENE KOMP DESIGN DIAGRAMS
VelociGene KOMP Design
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VelociGene Genotyping Strategy

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