12162 Chrdl1 Status: ES cell colonies screened / QC no positives
| Gene | Chrdl1 |
| Chromosome | X |
| Deletion Start | 143,313,957 |
| Deletion End | 143,311,399 |
| Deletion Size | 2,559 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome X
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 143,367,852 | 143,367,875 | TGAATGGAAACCACAGCATTAAGC |
| TUP | 143,367,825 | 143,367,848 | TTGGGAAGGAAAGCCTCATCGGCA |
| TUR | 143,367,756 | 143,367,774 | TGCAGCCTTTACCGTGGAC |
| TDF | 143,366,474 | 143,366,493 | GCAAAGCTGAATGCAAGGAC |
| TDP | 143,366,448 | 143,366,473 | TCACATGCCAGGGATGTCTCTGTACA |
| TDR | 143,366,396 | 143,366,419 | AGAAGAGAAAGGGTGGTGAGTAAC |
PCR Primers
| Primer | Sequence |
| SU | GTACTATGTCTCTTGACTTCC |
| SD | AACTCCCCTGTTACCTACTG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 120 bp | + | + | - |
| TDF | TDR | 98 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
