| Gene | Unc5b |
| Chromosome | 10 |
| Deletion Start | 60,783,268 |
| Deletion End | 60,765,110 |
| Deletion Size | 18,159 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 10
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 60,783,176 | 60,783,199 | CAGGACGCCTACATCGTAAAGAAC |
| TUP | 60,783,141 | 60,783,164 | ACTGCACTGCAGAGCCTTCCCTGC |
| TUR | 60,783,105 | 60,783,125 | ACCCACTCGCCATTACACTTG |
| TDF | 60,765,805 | 60,765,825 | CCAGGAGCTTACAGGGACTTC |
| TDP | 60,765,782 | 60,765,804 | CCCTGTCTGTGCTTCCCAGCTCC |
| TDR | 60,765,759 | 60,765,779 | GTTCCTTGAGCACGCCATTTC |
PCR Primers
| Primer | Sequence |
| SU | TCCTGGATGCCTCTGTGTC |
| SD | CAGTTTGCCTGAACTGAACTC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 95 bp | + | + | - |
| TDF | TDR | 67 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 12168A-G8 | VGB6 | C57BL/6NTac | |
| 12168A-H12 | VGB6 | C57BL/6NTac | |
