12169   Gdf3     Status: ES cell colonies screened / QC no positives

GeneGdf3
Chromosome6
Deletion Start122,609,979
Deletion End122,606,313
Deletion Size3,667
Genome Build38
MGI IDMGI:95686
NCBI Gene ID14562
BAC ID244P6
BAC LibrarybMQ
CassetteZEN-Ub1
Clones AvailableNo

Primer Genomic Coordinates on Chromosome 6

Please be aware that these oligonucleotides have been validated only by Taqman assay.

PrimerStartEndSequence
TUF122,609,569122,609,591TCTTGCACATTCTGTCTTGGTTC
TUP122,609,544122,609,568CAAGATCAGCCCAGAGGAAGGCACG
TUR122,609,517122,609,539GACTAGCGTGGAGAGGTGATAAG
TDF122,606,855122,606,875GGTCAGCTCCAGTTCAACCTG
TDP122,606,827122,606,849TGCGCTTAAGGATTGGAGCAGCA
TDR122,606,745122,606,764TTCTCGGGCTGGACAGTTAC

PCR Primers

PrimerSequence
SUTGTCTTCCTTTTCACTCACG
SDGCTACTATGCATAATGAGCAC
LacInRTTGACTGTAGCGGCTGATGTTG
LacInfGGTAAACTGGCTCGGATTAGGG
NeoInRTACTTTCTCGGCAGGAGCAAGGTG
NeoInFTTCGGCTATGACTGGGCACAACAG
LacInZRevGTCTGTCCTAGCTTCCTCACTG
NeoFwdTCATTCTCAGTATTGTTTTGCC

Predicted PCR Products

Forward PrimerReverse PrimerLengthWTHETKO
SULacZRev bp-++
TUFTUR75 bp++-
TDFTDR131 bp++-
NeoFwdSD bp-++
LacInfLacInR210 bp-++
NeoInfNeoInR282 bp-++
VELOCIGENE KOMP DESIGN DIAGRAMS
VelociGene KOMP Design
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VelociGene Genotyping Strategy

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