Information regarding availability, timelines and delivery of materials can be found at www.komp.org or www.knockoutmouse.org.

12171 Bmp8a Status: Germline transmission achieved

Gene	Bmp8a
Chromosome	4
Deletion Start	123,019,947
Deletion End	123,019,633
Deletion Size	315
Genome Build	37

MGI ID	MGI:104515
NCBI Gene ID	12163
BAC ID	304D3
BAC Library	bMQ
Cassette	ZEN-Ub1
Clones Available	Yes

Sequence Downloads and Strategy Information

Primer Genomic Coordinates on Chromosome 4

Please be aware that these oligonucleotides have been validated only by Taqman assay.

Primer	Start	End	Sequence
TUF	123,019,918	123,019,936	GGGCCACTCTGGCTATTGG
TUP	123,019,896	123,019,915	CTTGCTCTGTGCGCGCTGGG
TUR	123,019,800	123,019,818	AGCACCGCCAGGATTTCAC
TDF	123,019,718	123,019,737	CGCCCCTCTTCATGTTGGAC
TDP	123,019,692	123,019,713	ACCACGCCATGACCGATGACGA
TDR	123,019,661	123,019,680	GCCTAAGTGAGCCTGTGGTG

PCR Primers

Primer	Sequence
SU	TGCGGACACTCGCAAGTCA
SD	CCTGGACATCTCCAGATCC
LacInR	TTGACTGTAGCGGCTGATGTTG
LacInf	GGTAAACTGGCTCGGATTAGGG
NeoInR	TACTTTCTCGGCAGGAGCAAGGTG
NeoInF	TTCGGCTATGACTGGGCACAACAG
LacInZRev	GTCTGTCCTAGCTTCCTCACTG
NeoFwd	TCATTCTCAGTATTGTTTTGCC

Predicted PCR Products

Forward Primer	Reverse Primer	Length	WT	HET	KO
SU	LacZRev	bp	-	+	+
TUF	TUR	137 bp	+	+	-
TDF	TDR	77 bp	+	+	-
NeoFwd	SD	bp	-	+	+
LacInf	LacInR	210 bp	-	+	+
NeoInf	NeoInR	282 bp	-	+	+

Available Clones

ES Cell ordering information can be found at the KOMP Repository.

Clone Name	Parental ES Cell	Mouse Strain	Cassette Copy Number
12171A-F5	VGB6	C57BL/6NTac
12171A-E5	VGB6	C57BL/6NTac
12171A-H12	VGB6	C57BL/6NTac