12178 Gbx1 Status: ES cell colonies screened / QC no positives
| Gene | Gbx1 |
| Chromosome | 5 |
| Deletion Start | 24,526,813 |
| Deletion End | 24,504,538 |
| Deletion Size | 22,276 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 5
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 24,526,019 | 24,526,038 | ATCTGCTTGGAGCCCTGGAG |
| TUP | 24,525,997 | 24,526,018 | TGCCGGACTCCAGATCGTACGC |
| TUR | 24,525,962 | 24,525,982 | GCTTTGGGACCTGGAGATGTC |
| TDF | 24,504,784 | 24,504,803 | AGATCGCCCACGCCCTCAAG |
| TDP | 24,504,747 | 24,504,776 | AGGTGCAGGTGAAGATCTGGTTTCAGAACC |
| TDR | 24,504,694 | 24,504,715 | CCCAGAACGACTGCTCACATTG |
PCR Primers
| Primer | Sequence |
| SU | CTCCTAGGGATCGCCACTC |
| SD | AAAGCAGCAAAGCAGTAGGG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 77 bp | + | + | - |
| TDF | TDR | 110 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
