| Gene | Zbtb7b |
| Chromosome | 3 |
| Deletion Start | 89,381,506 |
| Deletion End | 89,379,526 |
| Deletion Size | 1,981 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 3
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 89,380,841 | 89,380,859 | GGCCCGAGCAAACCATCTG |
| TUP | 89,380,815 | 89,380,838 | CCCTGAGGCACCCACAGTACTCAC |
| TUR | 89,380,769 | 89,380,792 | ACCCAATCTACCAACCATCTCTTC |
| TDF | 89,380,430 | 89,380,454 | CCCAGTCTGTCACAAGATAATCCAC |
| TDP | 89,380,397 | 89,380,421 | CAAACTGCCTCGGCACATGAGGACC |
| TDR | 89,380,368 | 89,380,387 | TCACAGGCAAAGGGCTTCTC |
PCR Primers
| Primer | Sequence |
| SU | TGAGAACCAGGAAGGTGGTC |
| SD | GCATAGCCGAAGTGGGAC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 91 bp | + | + | - |
| TDF | TDR | 87 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 12179A-C2 | VGB6 | C57BL/6NTac | |
| 12179A-H4 | VGB6 | C57BL/6NTac | |
| 12179A-G7 | VGB6 | C57BL/6NTac | |
| 12179A-C10 | VGB6 | C57BL/6NTac | |
| 12179A-H5 | VGB6 | C57BL/6NTac | |
| 12179A-H10 | VGB6 | C57BL/6NTac | |
