12180 Radil Status: ES cell colonies screened / QC no positives
| Gene | Radil |
| Chromosome | 5 |
| Deletion Start | 142,543,936 |
| Deletion End | 142,543,407 |
| Deletion Size | 530 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 5
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 142,543,898 | 142,543,919 | GATCATGTCTCCTCCCACCAAG |
| TUP | 142,543,873 | 142,543,894 | AAGCTGAAGCGACAGAGCCAGC |
| TUR | 142,543,850 | 142,543,870 | CCGGGACAGCATTGTGGATAG |
| TDF | 142,543,646 | 142,543,665 | TGGTGAAAGAGGCACTGGAG |
| TDP | 142,543,621 | 142,543,645 | CGCTATGCCCTGGACCCTGAGTGTG |
| TDR | 142,543,583 | 142,543,606 | AGCTTGGCCCACAACATCACATAG |
PCR Primers
| Primer | Sequence |
| SU | ATGGCTAGATAGTGGGCGTG |
| SD | GATTCCTAACACGTCTGAGAG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 70 bp | + | + | - |
| TDF | TDR | 83 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
