12183 Tiam1 Status: Cancelled
| Gene | Tiam1 |
| Chromosome | 16 |
| Deletion Start | 89,898,564 |
| Deletion End | 89,877,041 |
| Deletion Size | 21,524 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 16
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 89,897,813 | 89,897,832 | AATGGAGGACCGAGGAACAG |
| TUP | 89,897,783 | 89,897,810 | TCTCAAGCTACTGCCGGAATTTGGTGTC |
| TUR | 89,897,743 | 89,897,765 | AGCCGGTGGCATCTTATGCTTTG |
| TDF | 89,877,289 | 89,877,310 | TGTTCAGCCTTTCCCTGATGAC |
| TDP | 89,877,263 | 89,877,285 | TGCCAGTGTCCCTTGGACGTCTT |
| TDR | 89,877,204 | 89,877,226 | GTGCAACCTGAGGAAACAGAATG |
PCR Primers
| Primer | Sequence |
| SU | TTGGCATCTACTCTTTGGGC |
| SD | CAACCAGTCCTTTCTGAGATG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 90 bp | + | + | - |
| TDF | TDR | 107 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
