12190 Mia1 Status: ES cell colonies screened / QC positives
| Gene | Mia1 |
| Chromosome | 7 |
| Deletion Start | 27,966,079 |
| Deletion End | 27,964,833 |
| Deletion Size | 1,247 |
| Genome Build | 37 |
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Primer Genomic Coordinates on Chromosome 7
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | | | CAGTTGCTGTCATTTCCTTCTCC |
| TUP | | | CCAGATCCTATCTCCATGGCTGTGGC |
| TUR | | | GTCAAGAAGCGGCAATCAGG |
| TDF | | | TGAGGGCTGCTGAGTTTACAG |
| TDP | | | CCCTGCCCAGTGCTGAGTGATCTC |
| TDR | | | ACTGCACCCTCTGCCATTTC |
PCR Primers
| Primer | Sequence |
| SU | TTGCTCAGTACCTGGGAACC |
| SD | TACGTTCTATGCGCTTCTCG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TCATTCTCAGTATTGTTTTGCC |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
