12191 Gsx2 Status: Cancelled
| Gene | Gsx2 |
| Chromosome | 5 |
| Deletion Start | 75,075,764 |
| Deletion End | 75,077,381 |
| Deletion Size | 1,618 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 5
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 75,076,452 | 75,076,473 | CACGGAGGTGGAAGTTAAGGAG |
| TUP | 75,076,475 | 75,076,498 | CAACTTCTTGGCGAGGCCTTGTGC |
| TUR | 75,076,521 | 75,076,541 | AGCCCAGATAACAGGCGACAC |
| TDF | 75,076,612 | 75,076,633 | GGACCTCAGTGTGGCATCTTAG |
| TDP | 75,076,636 | 75,076,662 | TCCCTGTTGGTTAAAGGCTGTCTGTGC |
| TDR | 75,076,663 | 75,076,680 | TAGCAGCCTCGGCCAAAG |
PCR Primers
| Primer | Sequence |
| SU | GTAGGCAGAAACTGGATCGC |
| SD | TTAATGGCAACTTCAATGATTTC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 90 bp | + | + | - |
| TDF | TDR | 69 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
