12192 Ubl4 Status: ES cell colonies screened / QC no positives
| Gene | Ubl4 |
| Chromosome | X |
| Deletion Start | 74,368,460 |
| Deletion End | 74,367,408 |
| Deletion Size | 1,053 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome X
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 74,368,201 | 74,368,223 | CCTGGTCTCGGATAAGCTGAATG |
| TUP | 74,368,181 | 74,368,200 | TCCCTGTGCGCCAGCAACGT |
| TUR | 74,368,125 | 74,368,143 | TCCCTGGATGGCTCCTTTC |
| TDF | 74,367,746 | 74,367,763 | AAAGTCCTGGCCCGTCAC |
| TDP | 74,367,720 | 74,367,745 | TTCAGTGTAGCAGATGCCAGCAGGGT |
| TDR | 74,367,692 | 74,367,715 | TCTTCTCACCCTCTGTAGTTGTTC |
PCR Primers
| Primer | Sequence |
| SU | ACGTGTTTGCATTGACTTGG |
| SD | AGATTTGCCAAGGGACACAC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 99 bp | + | + | - |
| TDF | TDR | 72 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
