12205 Brap Status: ES cell colonies screened / QC no positives
| Gene | Brap |
| Chromosome | 5 |
| Deletion Start | 121,660,770 |
| Deletion End | 121,685,587 |
| Deletion Size | 24,818 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 5
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 121,660,975 | 121,660,993 | CGATGCTGTGCGCCTTTCC |
| TUP | 121,660,994 | 121,661,017 | CAAGCTGGCAGTCAGACAGACCGG |
| TUR | 121,661,039 | 121,661,058 | TCCAGCCACTCACACCTAAC |
| TDF | 121,685,384 | 121,685,405 | CCAGGAAGGCCAGATCAACATC |
| TDP | 121,685,409 | 121,685,429 | ATGGCCTCAGCGCCCAACCCA |
| TDR | 121,685,503 | 121,685,523 | CCAGGAAGTCTCAGGGACAAG |
PCR Primers
| Primer | Sequence |
| SU | CTGCGGACCCATAGACTCTC |
| SD | ATGACATGCAAGCAGCTCTC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 84 bp | + | + | - |
| TDF | TDR | 140 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
