12214 Gabpb2 Status: Cancelled
| Gene | Gabpb2 |
| Chromosome | 3 |
| Deletion Start | 95,206,567 |
| Deletion End | 95,188,572 |
| Deletion Size | 17,996 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 3
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 95,206,486 | 95,206,507 | GTGAGGACACTGATGGCAAATG |
| TUP | 95,206,457 | 95,206,481 | TCCATTCACCACAGACTGGGTAAGC |
| TUR | 95,206,426 | 95,206,443 | TTGCTCGGGAAAGCACAG |
| TDF | 95,189,282 | 95,189,303 | GATGTGCTTTGGCTTGTAGTTC |
| TDP | 95,189,257 | 95,189,280 | AACTGTGCCTGCTGGTCAGGTTGC |
| TDR | 95,189,208 | 95,189,231 | TGGCAATTTCTCTTCTTCCTCTTC |
PCR Primers
| Primer | Sequence |
| SU | TAGGTACCCTTGTGCCTTGC |
| SD | GAGGAGGCTTCCTACAACTGC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 82 bp | + | + | - |
| TDF | TDR | 96 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
