12219 Araf Status: Cancelled
| Gene | Araf |
| Chromosome | X |
| Deletion Start | 20,849,990 |
| Deletion End | 20,860,142 |
| Deletion Size | 10,153 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome X
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 20,850,457 | 20,850,478 | CCCAGGTGACATCTTTCGTTTC |
| TUP | 20,850,482 | 20,850,511 | TCATTGACCATTGGAAGGTCTGCAATTCAC |
| TUR | 20,850,525 | 20,850,546 | GGTGACAGACTGAGGACTTGAG |
| TDF | 20,860,055 | 20,860,074 | GCACCCAGGCTGATGAGTTG |
| TDP | 20,860,077 | 20,860,097 | TGCTTGCCTTCTCAGCGCAGC |
| TDR | 20,860,102 | 20,860,123 | GGAGTGAGGTCTAAGGCACAAG |
PCR Primers
| Primer | Sequence |
| SU | CATTGTGTGCCTGAGACCTG |
| SD | ATAAACAAGCAAAGGCCCAG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 90 bp | + | + | - |
| TDF | TDR | 69 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
