| Gene | Col6a2 |
| Chromosome | 10 |
| Deletion Start | 76,615,202 |
| Deletion End | 76,596,196 |
| Deletion Size | 19,007 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 10
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 76,614,346 | 76,614,367 | GGACACCATCAACCGCATCATC |
| TUP | 76,614,316 | 76,614,341 | TCATGGTAAGCACCTGTGGTTCAGGC |
| TUR | 76,614,291 | 76,614,310 | TGGGTGGGCAGCATCCTTAG |
| TDF | 76,596,262 | 76,596,283 | TCCGGGAGAAAGACTTTGACAG |
| TDP | 76,596,241 | 76,596,261 | TCTGGCCCAGCCCAGCTTCTT |
| TDR | 76,596,201 | 76,596,219 | AGCATGGCGGTGCTAACAG |
PCR Primers
| Primer | Sequence |
| SU | TCCCAGTGAAGATCCTGGAG |
| SD | AGAAAGACAGAGCCTTGGGC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 77 bp | + | + | - |
| TDF | TDR | 83 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 12228B-D9 | VGB6 | C57BL/6NTac | |
| 12228B-E5 | VGB6 | C57BL/6NTac | |
| 12228B-H6 | VGB6 | C57BL/6NTac | |
| 12228C-G10 | VGB6 | C57BL/6NTac | |
| 12228C-E10 | VGB6 | C57BL/6NTac | |
