12231 Ran Status: ES cell colonies screened / QC no positives
| Gene | Ran |
| Chromosome | 5 |
| Deletion Start | 129,020,542 |
| Deletion End | 129,022,864 |
| Deletion Size | 2,323 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 5
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 129,020,669 | 129,020,692 | GTCTCTTGAATCTAGCTCGTCCTG |
| TUP | 129,020,704 | 129,020,726 | CGGCACCGGGAAGACAACCTTCG |
| TUR | 129,020,755 | 129,020,778 | CCGCACCTACCTACATACTTCTTC |
| TDF | 129,022,766 | 129,022,784 | TGCTCCAGGTTGCTCAGAC |
| TDP | 129,022,788 | 129,022,813 | TGCTCTCCCAGATGAGGATGATGACC |
| TDR | 129,022,819 | 129,022,837 | GGGCTCCAGCTTCACTTTC |
PCR Primers
| Primer | Sequence |
| SU | TCTTTCCAGCTCCAGTCGG |
| SD | AATATGGCACAAGCTCCCTC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 110 bp | + | + | - |
| TDF | TDR | 72 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
