12232 Hoxa5 Status: Cancelled
| Gene | Hoxa5 |
| Chromosome | 6 |
| Deletion Start | 52,204,347 |
| Deletion End | 52,202,562 |
| Deletion Size | 1,786 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 6
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 52,204,241 | 52,204,262 | TGAGCGAACAATTCAGGGACTC |
| TUP | 52,204,217 | 52,204,238 | CGAGCATGCACTCCGGCAGGTA |
| TUR | 52,204,185 | 52,204,207 | CGCTGAGATCCATGCCATTGTAG |
| TDF | 52,202,811 | 52,202,834 | CAGACAATATAGGTGGCCCAGAAG |
| TDP | 52,202,785 | 52,202,807 | AAAGGGCCCGGACGGCCTACACT |
| TDR | 52,202,731 | 52,202,751 | GGTCAGGTAGCGGTTGAAGTG |
PCR Primers
| Primer | Sequence |
| SU | ATTAGTGCACGAGTTTACCTC |
| SD | ATTGGCACTAAACGAGGTGG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 78 bp | + | + | - |
| TDF | TDR | 104 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
