12233 Pigr Status: Cancelled
| Gene | Pigr |
| Chromosome | 1 |
| Deletion Start | 130,834,432 |
| Deletion End | 130,850,994 |
| Deletion Size | 16,563 |
| Genome Build | 38 |
|
|
Primer Genomic Coordinates on Chromosome 1
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 130,834,444 | 130,834,464 | TTCACGCTCTTGGTAACTGTC |
| TUP | 130,834,468 | 130,834,493 | TCAGGTAGGTCTGATGGTCCCAACTC |
| TUR | 130,834,507 | 130,834,530 | AGAAACAAGGAGTCTAGGAGCAAC |
| TDF | 130,850,717 | 130,850,735 | TCCAGCAGAGATGGGAAAC |
| TDP | 130,850,744 | 130,850,770 | TCAGATGTAGGTCCTTCTCACCCGACA |
| TDR | 130,850,781 | 130,850,802 | GACTAGGGAGCAACAGAAGAGG |
PCR Primers
| Primer | Sequence |
| SU | AAAGAACACCATCCGTATCCC |
| SD | GCCTGGCTCTCAGCATTTAG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 87 bp | + | + | - |
| TDF | TDR | 86 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
