12236 Kng1 Status: Cancelled
| Gene | Kng1 |
| Chromosome | 16 |
| Deletion Start | 23,058,447 |
| Deletion End | 23,081,633 |
| Deletion Size | 23,187 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 16
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 23,058,609 | 23,058,627 | TTGCACCGAGTGATCGAGG |
| TUP | 23,058,637 | 23,058,665 | CGGTGAGTAACAGCTTTCCTGTGAAACCA |
| TUR | 23,028,666 | 23,028,687 | GCCTGAGAGCACCAATTACAAG |
| TDF | 23,080,981 | 23,081,005 | TTGCCTGGAAAAGAAGATATGACTG |
| TDP | 23,081,008 | 23,081,025 | TGAACTCACTGATAAGTC |
| TDR | 23,081,036 | 23,081,058 | CATGGGTATGGAGCTATGTGGAG |
PCR Primers
| Primer | Sequence |
| SU | CAGAGCCCTTTGTAACTGGG |
| SD | AGGTCACTGGGCACTTTCTG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 29962 bp | + | + | - |
| TDF | TDR | 78 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
