12239 Rlim Status: Cancelled
| Gene | Rlim |
| Chromosome | X |
| Deletion Start | 103,966,653 |
| Deletion End | 103,962,454 |
| Deletion Size | 4,200 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome X
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 103,966,616 | 103,966,638 | AACGATAAAGGAAGTGACCAGTC |
| TUP | 103,966,594 | 103,966,615 | TGCAGCTCAGCGCAGAAGTCAA |
| TUR | 103,966,564 | 103,966,585 | GAAAGCCTCTTCCCGATCCAAG |
| TDF | 103,962,975 | 103,962,995 | AGAGTCACGGAATGGAAGAGG |
| TDP | 103,962,951 | 103,962,973 | AGCTCTGGTGGCGGTAACAGCTC |
| TDR | 103,962,923 | 103,962,942 | GCTAGGACTTGAGCTGGAAC |
PCR Primers
| Primer | Sequence |
| SU | CCTCATGGTGGGACGTATTC |
| SD | CATGCCTCTCTTATGGCCTG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 75 bp | + | + | - |
| TDF | TDR | 73 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
