| Gene | Ube2r2 |
| Chromosome | 4 |
| Deletion Start | 41,136,073 |
| Deletion End | 41,174,121 |
| Deletion Size | 38,049 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 4
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 41,136,085 | 41,136,104 | ATGACCAGCTCGCAGAAAGC |
| TUP | 41,136,107 | 41,136,131 | TGATGCTTGAGCTGAAATCCCTGCA |
| TUR | 41,136,183 | 41,136,201 | GGCCACCTCCCAGTTGTAG |
| TDF | 41,173,425 | 41,173,443 | GGCCACACTCCGTCTTCTG |
| TDP | 41,173,445 | 41,173,471 | CCTCTGCAGTTACCAGGCAAACATGGT |
| TDR | 41,173,548 | 41,173,568 | CCCACCAAACCCAAGTCATTC |
PCR Primers
| Primer | Sequence |
| SU | TTCCTTCGCTTCCACCTCC |
| SD | ACACTTCAATTTAGAGGTGGG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 117 bp | + | + | - |
| TDF | TDR | 144 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 12240B-H10 | VGB6 | C57BL/6NTac | |
| 12240B-H5 | VGB6 | C57BL/6NTac | |
| 12240B-G9 | VGB6 | C57BL/6NTac | |
| 12240C-D12 | VGB6 | C57BL/6NTac | |
| 12240B-G7 | VGB6 | C57BL/6NTac | |
