12243 Pou3f3 Status: ES cell colonies screened / QC no positives
| Gene | Pou3f3 |
| Chromosome | 1 |
| Deletion Start | 42,697,149 |
| Deletion End | 42,698,869 |
| Deletion Size | 1,721 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 1
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 42,697,890 | 42,697,907 | GGAGCCCAGAGCCTGGTT |
| TUP | 42,697,908 | 42,697,925 | CACCCCGGGCTAGTGCGC |
| TUR | 42,697,938 | 42,697,956 | GATGATGCTCCGCCAACTC |
| TDF | 42,698,066 | 42,698,083 | TCACTCGGACGAGGACAC |
| TDP | 42,698,086 | 42,698,107 | CGACGTCTGACGACCTGGAGCA |
| TDR | 42,698,123 | 42,698,140 | TTGATGCGCCGCTGTTTG |
PCR Primers
| Primer | Sequence |
| SU | TGGAGCCAGCCGATCGCTC |
| SD | ACAGTACCTCAAGTGTGCAG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 67 bp | + | + | - |
| TDF | TDR | 75 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
