12247 Mtpn Status: Cancelled
| Gene | Mtpn |
| Chromosome | 6 |
| Deletion Start | 35,539,607 |
| Deletion End | 35,512,176 |
| Deletion Size | 27,432 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 6
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 35,539,264 | 35,539,286 | CCGGGTACTGCAATCTTTGAATG |
| TUP | 35,539,235 | 35,539,260 | CATTTCTCCATCTTGGCCTCGCACCG |
| TUR | 35,539,210 | 35,539,229 | TGCTCGGGTTTCCTTCTCAG |
| TDF | 35,512,249 | 35,512,270 | AGCCACTGACAACCAGGCAATC |
| TDP | 35,512,214 | 35,512,241 | TTCTCCAGTGATAGATGGATGGACGGAC |
| TDR | 35,512,176 | 35,512,193 | TGTGAGGCCACAGGAGAG |
PCR Primers
| Primer | Sequence |
| SU | CTCCTGAGGTAAGCAGTCGG |
| SD | GGAGCTGAGAAGGTCCAATG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 77 bp | + | + | - |
| TDF | TDR | 95 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
