12250 Gpr85 Status: Cancelled
| Gene | Gpr85 |
| Chromosome | 6 |
| Deletion Start | 13,836,900 |
| Deletion End | 13,835,742 |
| Deletion Size | 1,159 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 6
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 13,836,544 | 13,836,563 | GCATCAGCGTCACCAGATAC |
| TUP | 13,836,522 | 13,836,543 | TTAGCCATCGCCCATCACCGCT |
| TUR | 13,836,472 | 13,836,493 | CATGCAGATCACAGCCAAACAC |
| TDF | 13,836,223 | 13,836,243 | GTAGCAGCAGTGAGTCAGAAC |
| TDP | 13,836,199 | 13,836,222 | TGGACCTTTCATGGCCCTGGAGCT |
| TDR | 13,836,124 | 13,836,142 | CCTGATGCCCAGCAAGGTG |
PCR Primers
| Primer | Sequence |
| SU | CAATTGCAGCTGAAGCAAAC |
| SD | TCAACACATTTACCTCATGTCAAC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 92 bp | + | + | - |
| TDF | TDR | 120 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
