12252 Gpr30 Status: Cancelled
| Gene | Gpr30 |
| Chromosome | 5 |
| Deletion Start | 139,425,905 |
| Deletion End | 139,427,076 |
| Deletion Size | 1,172 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 5
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 139,426,079 | 139,426,098 | TTCCTCTCCTGCCTCTACAC |
| TUP | 139,426,104 | 139,426,131 | TCCTCTTTCCTATTGGCTTTGTGGGCAA |
| TUR | 139,426,168 | 139,426,187 | GGTCTGGGATGGTCATCTTC |
| TDF | 139,426,529 | 139,426,548 | GATGTCAGGGAGGTGCAGTG |
| TDP | 139,426,551 | 139,426,574 | TGGAGGTCACACTGGGCTTCATCA |
| TDR | 139,426,588 | 139,426,608 | AGGGAGTAGCAGAGGCCAATG |
PCR Primers
| Primer | Sequence |
| SU | GCTCCTTCAGGATGCTCAG |
| SD | AGGGAAGGTAGCCAGTTTGG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 109 bp | + | + | - |
| TDF | TDR | 80 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
