12256 Txn1 Status: Cancelled
| Gene | Txn1 |
| Chromosome | 4 |
| Deletion Start | 57,956,207 |
| Deletion End | 57,943,904 |
| Deletion Size | 12,304 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 4
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 57,955,787 | 57,955,808 | GGCCTTAGTTTCCCTCCATCAG |
| TUP | 57,955,766 | 57,955,786 | TGACCTGCCGCCTAGAGGACA |
| TUR | 57,955,718 | 57,955,739 | GTACTTAACTGGGACCCGAGAC |
| TDF | 57,944,881 | 57,944,901 | AGCCTGGAAGATTGCTGAGAG |
| TDP | 57,944,852 | 57,944,879 | TCAGTCAGAATGGGCTACAGGGTAGTAA |
| TDR | 57,944,818 | 57,944,839 | GAGTGCCAAGATCAAAGGTGTG |
PCR Primers
| Primer | Sequence |
| SU | TAAGGTTCTTGTTCGGCAGC |
| SD | TCACACTCATGGTCTGGACC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 91 bp | + | + | - |
| TDF | TDR | 84 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
