12269 Cldn7 Status: ES cell colonies screened / QC no positives
| Gene | Cldn7 |
| Chromosome | 11 |
| Deletion Start | 69,965,930 |
| Deletion End | 69,967,765 |
| Deletion Size | 1,836 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 11
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 69,966,026 | 69,966,043 | TCCTATGCGGGCGACAAC |
| TUP | 69,966,045 | 69,966,068 | TCATCACAGCCCAGGCCATGTACA |
| TUR | 69,966,095 | 69,966,114 | CTCATCATGCCGGTGCTCTG |
| TDF | 69,966,894 | 69,966,914 | AAGGCTACCTGCCATTTCCAG |
| TDP | 69,966,915 | 69,966,936 | CAATGACCGCCGCCTTCCTCCC |
| TDR | 69,966,995 | 69,967,015 | GTGGCGACAAACATGGCTAAG |
PCR Primers
| Primer | Sequence |
| SU | AAGGGCCCGCATACTTTC |
| SD | CTGCCCTACACATACCCACC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 89 bp | + | + | - |
| TDF | TDR | 122 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
