12270 Adamts1 Status: ES cell colonies screened / QC no positives
| Gene | Adamts1 |
| Chromosome | 16 |
| Deletion Start | 85,802,707 |
| Deletion End | 85,795,303 |
| Deletion Size | 7,405 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 16
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 85,802,315 | 85,802,334 | ACCTGGCTCACTGCTTCTAC |
| TUP | 85,802,292 | 85,802,314 | TCTGGCACGGTGAACGGTGATCC |
| TUR | 85,802,245 | 85,802,263 | AGGCACCACGCACACCTTC |
| TDF | 85,795,872 | 85,795,890 | GGCTGCGCTGGAAAGAATC |
| TDP | 85,795,845 | 85,795,871 | CGCAGCTTTAGTCCACTCAAAGAACCC |
| TDR | 85,795,810 | 85,795,832 | AGAGCATGGCCTACCATAAGAAC |
PCR Primers
| Primer | Sequence |
| SU | CTCCGAGCTAGGTGCTATCG |
| SD | AACCAGCGACTTCGAATAATG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 90 bp | + | + | - |
| TDF | TDR | 81 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
