12274 Gpr3 Status: Cancelled
| Gene | Gpr3 |
| Chromosome | 4 |
| Deletion Start | 133,211,356 |
| Deletion End | 133,210,366 |
| Deletion Size | 991 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 4
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 133,211,324 | 133,211,345 | AGGAAGCTCTATGGCCTGGTTC |
| TUP | 133,211,301 | 133,211,323 | TCAGCTGGCTCAGGCAGTGTGAA |
| TUR | 133,211,263 | 133,211,281 | GGCCTGTGGGTTCCTCTAC |
| TDF | 133,210,581 | 133,210,598 | CGCCTTTGCTGCCTGTTG |
| TDP | 133,210,555 | 133,210,578 | TGCCCTTCACTGTCTACTGCCTCC |
| TDR | 133,210,510 | 133,210,533 | GCAGGGTAAGATAGGTGTAGAGAC |
PCR Primers
| Primer | Sequence |
| SU | TTCTTGACGTGTTTCCCTCC |
| SD | CCTCTGACTCTGGAGCTTCG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 83 bp | + | + | - |
| TDF | TDR | 89 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
