12275 Synpo2l Status: Cancelled
| Gene | Synpo2l |
| Chromosome | 14 |
| Deletion Start | 20,668,192 |
| Deletion End | 20,660,290 |
| Deletion Size | 7,903 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 14
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 20,667,423 | 20,667,444 | TCATACAGTGCAGTGAGAACTC |
| TUP | 20,667,397 | 20,667,418 | AGCCTGGCATCACAGTCTCCCA |
| TUR | 20,667,373 | 20,667,396 | GACTGAGGTAACCCAGCTATATTC |
| TDF | 20,661,068 | 20,661,088 | CAACGCCTAAGACTCCACCTC |
| TDP | 20,661,044 | 20,661,066 | CGTGGCTCCTAAACCTGGGTCGC |
| TDR | 20,660,987 | 20,661,006 | GCTCAGGGATTCCCACCATC |
PCR Primers
| Primer | Sequence |
| SU | TCATGATCCTTGGAGGAAGG |
| SD | AACCTAGGGAAAGCGGAGTG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 72 bp | + | + | - |
| TDF | TDR | 102 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
