12276 Ocrl Status: ES cell colonies screened / QC one positive
| Gene | Ocrl |
| Chromosome | X |
| Deletion Start | 47,912,656 |
| Deletion End | 47,963,615 |
| Deletion Size | 50,960 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome X
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 47,913,235 | 47,913,252 | GTGGCTCAGTGGATGGAC |
| TUP | 47,913,253 | 47,913,277 | TCAATGGACAGGAAAGCAGGAAGCC |
| TUR | 47,913,281 | 47,913,305 | CAGACAGGGTGAAGGAACTAAGAAC |
| TDF | 47,963,462 | 47,963,482 | ACCCTGTTCAGTAGCCTTCTC |
| TDP | 47,963,485 | 47,963,512 | AAGACCTCCACCAAACCTCATGACAAGA |
| TDR | 47,963,519 | 47,963,537 | CATGCTGGCGGTCATTTGG |
PCR Primers
| Primer | Sequence |
| SU | TGAAGAGGGACCTGCTATGG |
| SD | ACAGGAAGGCCTCTCAATCC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 71 bp | + | + | - |
| TDF | TDR | 76 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
