12278 Rab33a Status: Cancelled
| Gene | Rab33a |
| Chromosome | X |
| Deletion Start | 48,519,604 |
| Deletion End | 48,530,133 |
| Deletion Size | 10,530 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome X
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 48,520,373 | 48,520,394 | GAGCTGACTTGAGATGGAGGTG |
| TUP | 48,520,396 | 48,520,423 | CATGGAGCATGGTTATGTCACAAAGCCC |
| TUR | 48,520,443 | 48,520,460 | GAAGAGCCAGCAGGTGAC |
| TDF | 48,530,016 | 48,530,034 | TGTACCGTGATGCCGAGAG |
| TDP | 48,530,039 | 48,530,065 | CAAGGCAAGGTGCAGAAACTGGAGTTC |
| TDR | 48,530,089 | 48,530,109 | TGGCTTCAACAAGGACAGGAG |
PCR Primers
| Primer | Sequence |
| SU | TCCACACTCCTCATCCCTTC |
| SD | ATCCACATCTCATCTCACAAC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 88 bp | + | + | - |
| TDF | TDR | 94 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
