12280 Gsc Status: Cancelled
| Gene | Gsc |
| Chromosome | 12 |
| Deletion Start | 104,473,112 |
| Deletion End | 104,471,395 |
| Deletion Size | 1,718 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 12
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 104,472,423 | 104,472,443 | GCCTGAACTGGCTTTTGGATG |
| TUP | 104,472,401 | 104,472,422 | TGTGCAGGCAGACGCTCCTTCC |
| TUR | 104,472,380 | 104,472,398 | AGACCAGGGATGCCCAGAC |
| TDF | 104,472,105 | 104,472,122 | GCACCGCACCATCTTCAC |
| TDP | 104,472,078 | 104,472,102 | ATGAGCAGCTCGAAGCCCTGGAGAA |
| TDR | 104,472,047 | 104,472,067 | CACGTCTGGGTACTTCGTCTC |
PCR Primers
| Primer | Sequence |
| SU | ACCCGCGGGTTGAGTTCAG |
| SD | GAAGACCCAGGATACCCAGC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 64 bp | + | + | - |
| TDF | TDR | 76 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
